目的 建立測(cè)定金銀花藥材中新綠原酸、綠原酸、隱綠原酸、咖啡酸、異綠原酸A、異綠原酸B、異綠原酸C、斷氧化馬錢(qián)子苷8種成分的HPLC方法。方法 采用RP-HPLC法，色譜柱為L(zhǎng)una 5 μ C18柱（250 mm×4.6 mm，5 μm）；流動(dòng)相為甲醇-0.1%磷酸水溶液，梯度洗脫，0～20 min，12%～30% A；20～60 min，30%～50% A，體積流量1.0 mL/min；檢測(cè)波長(zhǎng)237、324 nm，柱溫30 ℃。結(jié)果 8種成分均達(dá)到基線分離，各成分均有較寬的線性范圍和良好的線性關(guān)系（r＞0.999 9）；回收率在98.72%～102.50%。結(jié)論 本方法準(zhǔn)確靈敏、重復(fù)性好，能較全面地評(píng)價(jià)金銀花藥材的質(zhì)量。

Objective To establish an HPLC method for simultaneously determining eight components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, and secoxyloganin in Lonicera japonica. Methods An RP-HPLC method was established with Luna C18 (250 mm × 4.6 mm, 5 μm) column by a gradient elution using methanol and 0.1% phosphoric acid; The flow rate was 1.0 mL/min with double wavelength (237 and 324 nm), and the column temperature was 30 ℃. Results Baseline of eight components was separated; Each component had a wide linear range and a good linear relationship (r > 0.999 8); the recoveries were 98.72% to 102.50%. Conclusion The method is accurate, sensitive, reliable, and with good reproducibility. It could be used for the quality control of L. japonica.

國(guó)家科技部國(guó)家重大新藥創(chuàng)制專(zhuān)項(xiàng)“現(xiàn)代中藥創(chuàng)新集群與數(shù)字制藥技術(shù)平臺(tái)”（2013ZX09402203）