目的 研究鹿藿Rhynchosia volubilis的化學(xué)成分。方法 采用硅膠、ODS、Toyopearl HW-40C、Sephadex LH-20柱色譜以及半制備HPLC等方法進(jìn)行純化，根據(jù)理化性質(zhì)及波譜數(shù)據(jù)對(duì)化合物進(jìn)行結(jié)構(gòu)鑒定。結(jié)果 從鹿藿石油醚提取物中分離得到13個(gè)化合物，分別鑒定為（-）-sigmoidin E（1）、lupinifolin（2）、precatorin B（3）、木豆酮（4）、槐花多糖B（5）、5，3'-二羥基-4'-甲氧基-5'-γ，γ-二甲基烯丙基-2″，2″-二甲基吡喃并[5，6：6，7]異黃烷酮（6）、染料木素（7）、甘草異黃酮A（8）、erylatissin B（9）、neo-bavaisoflavone（10）、羽扇豆醇（11）、樺木醛（12）、穿貝海綿甾醇（13）?；衔?b>1～10均為含異戊烯基的黃酮類，其中化合物1、2為二氫黃酮、3～6為二氫異黃酮、7～10為異黃酮?；衔?b>11～12為羽扇豆烷型三萜，化合物13為甾醇類。結(jié)論 化合物1、5～6、8～10、12、13均為首次從鹿藿屬植物中分離得到，化合物1～3、5～13均為首次從該植物中分離得到。

Objective To study the chemical constituents from Rhynchosia volubilis. Methods The compounds were isolated and purified by a combination of various chromatographic techniques including silica gel, ODS, Toyopearl HW-40C, Sephadex LH-20, and semi-preparative HPLC chromatography. Their structures were identified by physicochemical properties and spectroscopic data. Results Thirteen compounds were isolated from the petroleum ether extracts of R. volubilis and their structures were elucidated as (-)-sigmoidin E (1), lupinifolin (2), precatorin B (3), cajanone (4), sophoraisoflavanone B (5), 5,3'-dihydroxy-4'-methoxy-5'- γ,γ-dimethylallyl-2″,2″-dimethylpyrano[5,6:6,7] isoflavanone (6), genistein (7), licoisoflavone A (8), erylatissin B (9), neo-bavaisoflavone (10), lupeol (11), betulinic aldehyde (12), and clionasterol (13). Compounds 1-10 were all prenylated flavonoids, of which compounds 1-2 were dihydroflavones, compounds 3-6 were dihydroisoflavones, and compounds 7-10 were isoflavones. Compounds 11-12 were lupine triterpenoids, and compounds 13 was a sterol. Conclusion Compounds 1, 5-6, 8-10, 12 and 13 are isolated from the genus for the first time, while compounds 1-3 and 5-13 are separated from this plant for the first time.

R284.1

國(guó)家重點(diǎn)研發(fā)計(jì)劃——“生殖健康及重大出生缺陷防控研究”重大專項(xiàng)資助項(xiàng)目（2016YFC1000905）