50)分別為9.46、11.86、8.73 μmol/L。結(jié)論 所有化合物均為首次從川芎莖葉中分離得到,化合物6和8為首次從川芎中分離。受試化合物均有舒張血管活性,且二聚體的活性優(yōu)于單苯酞。;Objective To study the phthalides with vasodilating activity of alcohol extracts from stems and leaves of Ligusticum chuanxiong. Methods Compounds were isolated by extensive chromatographic techniques including MCI, silica gel, Sephadex LH-20, ODS, and semi-preparative HPLC. The vasodilating effect of phthalide and dimer phthalide on thoracic aortic ring in vitro was investigated. Results Ten phthalides were isolated and identified, and their structures were elucidated as Z-3-butylidenephthalide (1), senkyunolide-E (2), Z-senkyunolide-H (3), (3Z,7β)-3-butenyl-7-hydroxy-4,5,6,7-tetrahydrophthalide (4), neoligustilide (5); Z,Z'-3.3'a,7.7'a-diligustilide (6), 3Z,3Z'-6,8',7,3'-diligustilide (7), Z-tokinolide A (8), (3'Z)-(3S,8R,3a'S,6'R)-4,5-dehydro- 3.3'a,8.6'-diligustilide (9), and (3Z)-(3aR,6S,3'R,8'S)-3a.8',6.3'-diligustilide (10). Compound 1, 6-9 significantly reduced the strain of thoracic aortic ring in KCl preconstricted rats. The diastolic rate of compounds 6-8 were 60%, 52% and 70% at the highest concentrations (12 μmol/L), respectively. The EC50 was 9.46, 11.86 and 8.73 μmol/L, respectively. Conclusion All compounds are firstly isolated from the stems and leaves of L. chuanxiong. Compounds 6, 8 are firstly isolated from L. chuanxiong. The test compounds could dilate blood vessels, and the activity of dimer phthalide was better than that of phthalide."/>