目的 旨在建立基于體外抗血小板聚集的小金丸生物效價(jià)檢測(cè)方法，評(píng)價(jià)其質(zhì)量一致性，篩選小金丸組方中發(fā)揮活血作用的中藥。方法 小金丸及其組方中的10味中藥（人工麝香、木鱉子、制草烏、楓香脂、醋乳香、醋沒藥、五靈脂、酒當(dāng)歸、地龍、京墨）40%甲醇超聲提取，以提取物為供試品通過血小板聚集儀測(cè)定其抗血小板聚集率。大鼠腹主動(dòng)脈取血，制備富血小板血漿（PRP）和乏血小板血漿（PPP），用二磷酸腺苷（ADP）誘導(dǎo)血小板聚集，以阿魏酸鈉作為標(biāo)準(zhǔn)對(duì)照物質(zhì)，根據(jù)簡(jiǎn)化概念單位法計(jì)算小金丸的抗血小板聚集生物效價(jià)。結(jié)果 不同廠家、不同批次間小金丸的抗血小板聚集生物效價(jià)在0.598～1.338 U/mg。小金丸組方中地龍、五靈脂和木鱉子抑制血小板聚集作用較強(qiáng)，抑制率分別為70.87%、31.83%、67.52%。結(jié)論 不同廠家、不同批次小金丸質(zhì)量一致性較差，地龍、五靈脂和木鱉子可能是小金丸組方中發(fā)揮活血作用的關(guān)鍵中藥。

Objective To establish a biological potency assay for Xiaojin Pills against platelet aggregation in vitro, evaluate the quality consistency of Xiaojin Pills, and screen traditioanal Chinese medicines which play the role of promoting blood circulation in Xiaojin Pills. Methods Xiaojin Pills and ten Chinese medicines[artificial musk, Momordica cochinchinensis, Aconitum kusnezoffii, Liquidambar formosana, Boswellia carterii, Commiphora myrrha, Faeces Trogopterori, Angelica sinensis, Pheretima aspergillum, Fragrant Ink] in its formula were extracted by ultrasound in 40% methanol. The antiplatelet aggregation rate of the extract was measured by platelet aggregation meter. The platelet-rich plasma (PRP) and platelet-poor plasma (PPP) were prepared from abdominal aorta of rats. The platelet aggregation was induced by adenosine diphosphate (ADP). With sodium ferulate as a standard reference material, the biological potency of antiplatelet aggregation of Xiaojin Pills was calculated by the simplified probit principle. Results The results showed that the biological potency of Xiaojin Pills was between 0.598 and 1.338 U/mg among different manufacturers and batches. In Xiaojin Pills group, Pheretima, Faeces Trogopterori, and Momordicae Semen had stronger inhibitory effects on platelet aggregation with inhibition rates of 70.87%, 31.83% and 67.52%, respectively. Conclusion The quality consistency of Xiaojin Pills from different manufacturers and batches is poor, and Pheretima, Faeces Trogopterori, and Momordicae Semen may be the key drugs for Xiaojin Pills to play the role of promoting blood circulation.

R283.6

國(guó)家自然科學(xué)基金資助項(xiàng)目（81873232）；國(guó)家自然科學(xué)基金資助項(xiàng)目（81960696）；成都中醫(yī)藥大學(xué)中藥學(xué)學(xué)科特色創(chuàng)新科研團(tuán)隊(duì)（CXTD2018006）