目的 研究黨參遠(yuǎn)志散微乳對(duì)阿爾茨海默?。ˋD）模型小鼠腦組織中乙酰膽堿（Ach）和乙酰膽堿酯酶（AchE）水平及腦組織中AchE和乙酰膽堿轉(zhuǎn)移酶（ChAT）蛋白表達(dá)的影響，運(yùn)用膽堿能學(xué)說(shuō)分析作用機(jī)制。方法 小鼠隨機(jī)分為對(duì)照組、模型組、生物相容性微乳組、腦復(fù)康組、黨參遠(yuǎn)志散水提液組和黨參遠(yuǎn)志散微乳組，每組15只。除對(duì)照組外，其余組均ip D-半乳糖（D-gal）和NaNO₂制備AD小鼠模型，分別ig蒸餾水、生物相容性微乳、腦復(fù)康、黨參遠(yuǎn)志散水提液和黨參遠(yuǎn)志散微乳6周。采用水迷宮實(shí)驗(yàn)進(jìn)行行為學(xué)考察；酶聯(lián)免疫分析測(cè)定腦組織中Ach和AchE水平；HE染色光鏡觀察海馬CA1區(qū)和皮層的病理變化；免疫組織化學(xué)染色法檢測(cè)海馬CA1區(qū)和皮層的ChAT、AchE蛋白表達(dá)。結(jié)果 與模型組比較，黨參遠(yuǎn)志散微乳組小鼠學(xué)習(xí)記憶能力明顯提高（P<0.05），腦組織中Ach水平明顯升高（P<0.01），AchE水平明顯降低（P<0.01），海馬CA1區(qū)和皮層的病理結(jié)構(gòu)改變明顯改善，ChAT的蛋白陽(yáng)性表達(dá)明顯上升（P<0.01），AchE蛋白陽(yáng)性表達(dá)明顯下降（P<0.01）。結(jié)論 黨參遠(yuǎn)志散微乳可通過(guò)上調(diào)小鼠海馬CA1區(qū)和皮層中ChAT表達(dá)和抑制AchE表達(dá)而發(fā)揮抗AD作用。

Objective To observe the effects of Dangshen Yuanzhi Powder microemulsion on protein expression of AchE and ChAT, and the contents of Ach and AchE in brain tissue of Alzheimer's disease model mice and analyze its mechanism based on cholinergic theory. Methods Mice were divided into control group, model group, BME group, Piracetam group, water extractant group and microemulsion extractant group, with 15 mice in each group. Alzheimer's disease mice model was established by intraperitoneal injection with D-gal and NaNO₂. Meanwhile, each group was given by gavage for six weeks with distilled water, BME, Piracetam tablets, water extractant and microemulsion extractant. Water maze was used to conduct behavior study. Contents of Ach and AchE in brain tissue were also measured. HE staining was used to observe pathological changes and IHC staining was used to detect expression of AchE and ChAT in hippocampal CA1 region and cerebral cortex of mice. Results Compared with the model group, learning and memory abilities of mice were significantly improved (P<0.05), content of Ach was significantly improved (P<0.01), content of AchE was significantly decreased (P<0.01), pathological changes of hippocampal CA1 region and cerebral cortex were improved, AChE expression was significantly downregulated (P<0.01), and ChAT expression was markedly upregulated (P<0.01) in microemulsion extractant group. Conclusion Dangshen Yuanzhi Powder microemulsion can control the progression of Alzheimer's disease by upregulating ChAT expression and inhibiting AChE expression in hippocampal CA1 region and cerebral cortex of mice.

R285.5

山西省“1331工程”工程（技術(shù)）研究中心中藥微乳與生物新制劑研發(fā)；山西中醫(yī)藥大學(xué)校級(jí)科技創(chuàng)新團(tuán)隊(duì)（2018TD-007）；晉藥綜合開發(fā)利用協(xié)同創(chuàng)新中心項(xiàng)目（2017-JYXT-19）；山西省應(yīng)用基礎(chǔ)研究計(jì)劃項(xiàng)目（201901D211539）；山西省中醫(yī)藥管理局科研項(xiàng)目（2019ZYYC022）