目的 探究人參皂苷Rg₁對APP/PS1雙轉(zhuǎn)基因模型小鼠認(rèn)知障礙、氧化應(yīng)激損傷、炎癥反應(yīng)、Aβ堆積和神經(jīng)細(xì)胞凋亡的改善作用。方法 采用Morris水迷宮實驗，對APP/PS1模型小鼠的認(rèn)知功能進行評價；采用試劑盒和ELISA法對各組小鼠氧化損傷和炎癥相關(guān)指標(biāo)進行測定；采用HE染色和Western blotting法對各組小鼠海馬組織神經(jīng)元凋亡及凋亡相關(guān)蛋白進行定量分析。結(jié)果 人參皂苷Rg₁能夠顯著改善APP/PS1雙轉(zhuǎn)基因模型小鼠的認(rèn)知功能，降低Aβ堆積及丙二醛（MDA）水平，提高超氧化物歧化酶（SOD）、谷胱甘肽過氧化物酶（GSH-P_X）、過氧化氫酶（CAT）活性。此外，人參皂苷Rg₁還可通過顯著性降低APP/PS1小鼠血清腫瘤壞死因子-α（TNF-α）、γ干擾素（IFN-γ）、白細(xì)胞介素-1β（IL-1β）和IL-6水平，增加IL-2水平，從而抑制神經(jīng)炎癥反應(yīng)。HE染色顯示人參皂苷Rg₁能降低海馬細(xì)胞的凋亡狀態(tài)。人參皂苷Rg₁還可以顯著升高海馬中Bcl-2/Bax的值，并顯著降低Caspase-3、Caspase-9和Cyt-c蛋白的表達水平。結(jié)論 人參皂苷Rg₁能夠顯著改善APP/PS1模型小鼠的氧化應(yīng)激狀態(tài)，減輕炎癥反應(yīng)，抑制神經(jīng)元凋亡和改善認(rèn)知功能。

Objective To investigate the effects of ginsenoside Rg₁ on cognitive impairment, oxidative stress injury, inflammation, accumulation of A beta and neuronal apoptosis in APP/PS1 mice. Methods Morris water maze test was used to evaluate the cognitive function of APP/PS1 mice. The oxidative damage and inflammation related indexes of APP/PS1 mice in each group were measured by kit and Elisa. The apoptotic and apoptotic proteins of hippocampal neurons in each group were quantified by HE staining and Western blot. Results The results showed that Rg₁ could significantly improve the cognitive function of mice, reduce the accumulation of Aβ and the content of malondialdehyde (MDA), and increase the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT). In addition, Rg₁ could significantly reduce the serum levels of TNF-α, INF-γ, IL-1β and IL-6 in APP/PS1 mice, and increase the level of IL-2, thereby inhibiting the neuroinflammatory response. HE staining showed that Rg₁ could reduce the apoptotic state of hippocampal cells. Rg₁ also significantly increased the expression of Bcl-2/Bax protein ratio, and reduced the expression of Caspase-3, Caspase-9 and Cyt-c protein in hippocampus. Conclusion Rg₁ can significantly improve oxidative stress, reduce inflammatory response, inhibit neuronal apoptosis and improve cognitive function in APP/PS1 mice.

R285.5

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