目的 研究煮炸鹿茸加工過程中晚期糖基化終產(chǎn)物的生成規(guī)律及動力學參數(shù)。方法 通過構建葡萄糖與賴氨酸模擬煮炸鹿茸加工過程美拉德（Maillard）反應體系，分別采用紫外-可見分光光度法和UPLC-MS/MS法測定體系褐變指數(shù)和典型晚期糖基化終產(chǎn)物（羧甲基賴氨酸和羧乙基賴氨酸）含量變化，探討晚期糖基化終產(chǎn)物的生成規(guī)律和動力學參數(shù)。結果 鹿茸加工過程中煮炸時發(fā)生褐變反應，生成羧甲基賴氨酸和羧乙基賴氨酸反應的活化能分別為5.07、40.44、78.47 kJ/mol，且均為零級反應；烘烤時相應反應的活化能分別為6.72、89.34、164.77 kJ/mol，也均為零級反應。相對于生成羧甲基賴氨酸而言，生成羧乙基賴氨酸所需能量更高，反應更難發(fā)生。結論 烘烤過程溫度變化對晚期糖基化終產(chǎn)物的動力學參數(shù)影響顯著高于煮炸過程，長時間較高溫度的烘烤使鹿茸中產(chǎn)生了較多的晚期糖基化終產(chǎn)物，這些結果為鹿茸加工過程中晚期糖基化終產(chǎn)物的阻斷、抑制策略提供了理論基礎，對生產(chǎn)綠色安全的鹿茸及加強中藥安全具有重要意義。

Objective To study the formation regularity and kinetic parameters of advanced glycation end-products during the processing of boiled Cervi Cornu Pantotrichum (CCP). Methods UV-visible spectrophotometry and UPLC-MS/MS method were used to determine the change of browning index and content of typical advanced glycation end-products, N^ε-(carboxymethyl) lysine and N^ε-(carboxyethyl) lysine, of the processing system of simulated boiled CCP. The formation regularity and kinetic parameters of advanced glycation end-products during the processing of boiled CCP were discussed by constructing glucose and lysine to simulate the Maillard reaction system of CCP processing. Results The activation energy of browning reaction, N^ε-(carboxymethyl) lysine and N^ε-(carboxyethyl) lysine reaction during processing of boiled CCP were 5.07, 40.44 and 78.47 kJ/mol, respectively, and all of them were zero-order kinetics. The activation energies of the above reactions in the baking process were 6.72, 89.34 and 164.77 kJ/mol, respectively, and all of them were zero-order kinetics. Compared to the formation of N^ε-(carboxymethyl) lysine, the formation of N^ε-(carboxyethyl) lysine required higher activation energy and was more difficult to occur. Conclusion The temperature changed in the baking process has a significantly higher effect on the kinetic parameters of the advanced glycation end-products than in the boiling process. Long-term higher baking temperature resulted in more advanced glycation end-products produced in the boiled CCP. This study provides a solid theoretical basis for the blocking and inhibition strategies of advanced glycation end-products in the processing of CCP, which is also a great significance for the production of green safety CCP and strengthening the safety of traditional Chinese medicine.

R283.1

國家重點研發(fā)計劃項目（2018YFC1706604）；國家重點研發(fā)計劃項目（2018YFC1706605）；中國農(nóng)業(yè)科學院科技創(chuàng)新工程項目（CAAS-ASTIP-2016-ISAPS）；吉林省科技發(fā)展計劃項目（20180201076YY）；中國農(nóng)業(yè)科學院特產(chǎn)研究所基本科研業(yè)務費（1610342019010）