目的 對狼毒大戟Euphorbia fischeriana中的酚酸類化學成分進行分離和結(jié)構(gòu)鑒定，并評價化合物對α-葡萄糖苷酶的抑制活性。方法 采用多種色譜技術(shù)進行分離純化，通過紅外、高分辨質(zhì)譜及核磁共振等波譜學手段鑒定化合物結(jié)構(gòu)，采用PNPG法測定α-葡萄糖苷酶抑制活性。結(jié)果 從狼毒大戟的根部分離并鑒定了8個酚酸類化合物，分別為苯甲酸甲酯-4-O-α-L-呋喃阿拉伯糖基-(1→6)-β-D-葡萄糖苷（1）、苯乙基-6-O-α-L-呋喃阿拉伯糖基-β-D-葡萄糖苷（2）、東莨菪苷（3）、corilagin（4）、6-羥基-2-甲氧基苯乙酮-4-O-β-D-葡萄糖苷（5）、6-羥基-2-甲氧基苯乙酮-4-O-α-L-阿拉伯糖-(1→6)-β-D-葡萄糖苷（6）、6-羥基-2-甲氧基苯乙酮-4-O-β-D-木糖-(1→6)-β-D-葡萄糖苷（7）、erythroxyloside B（8）。結(jié)論 化合物1為新化合物，命名為狼毒酚苷A（euphfischcoside A），化合物2～4為首次從大戟屬中分離得到?；衔?b>4和8對α-葡萄糖苷酶的半數(shù)抑制濃度（median inhibition concentration，IC₅₀）值分別為（7.44±3.95）μmol/L和（134.40±30.27）μmol/L。

Objective To investigate the chemical constituents of the roots of Euphorbiafischeriana and evaluate their α-glucosidase inhibitory activity. Methods A variety of chromatographic techniques were applied for the chemical separation and purification. Their structures were elucidated by a combination of IR, HR-ESI-MS and NMR spectra. PNPG method was performed to evaluate the α-glucosidase inhibitory activity. Results Eight phenolic compounds were isolated from the roots of E. fischeriana. Their structures were identified as methyl benzoate-4-O-α-L-arabinofuranosyl-(1→6)-β-D-glucopyranoside (1), phenethyl 6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (2), scopoletin (3), corilagin (4), 6-hydroxy-2-methoxyacetophenone-4-O-β-D-glucopyranoside (5), 6-hydroxy-2-methoxyacetophenone-4-O-α-L-arabinofuranosyl-(1→6)-β-D-glucopyranoside (6), 6-hydroxy-2-methoxyacetophenone-4-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside (7), erythroxyloside B (8). Conclusions Compound 1 is a new compound and named as euphfischcoside A, compounds 2―4 were isolated from E. fischeriana for the first time. Compounds 4 and 8 exhibit the considerable inhibition on α-glucosidase with the IC₅₀ values of (7.44 ±3.95) μmol/L and (134.40 ±30.27) μmol/L, respectively.

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國家自然科學基金項目（82304872）;北京市自然科學基金青年項目（7234403）;國家自然科學基金項目（81872761）