目的 研究丹參Salvia miltiorrhiza內(nèi)生真菌細(xì)鏈格孢Alternaria malvae的次生代謝產(chǎn)物及生物活性。方法 采用正相柱色譜、MPLC-ODS、MCI、Sephadex LH-20，結(jié)合制備型高效液相等色譜手段進(jìn)行分離純化，利用核磁共振和質(zhì)譜技術(shù)對單體化合物進(jìn)行鑒定，通過檢測脂多糖（lipopolysaccharide，LPS）誘導(dǎo)小鼠巨噬細(xì)胞（RAW264.7）一氧化氮（NO）的釋放，評價化合物的抗炎活性。結(jié)果 從細(xì)鏈格孢的大米發(fā)酵產(chǎn)物中分離得到7個化合物，分別鑒定為3-甲氧基細(xì)鏈格孢內(nèi)酯B（1）、4'-表細(xì)鏈格孢烯（2）、(＋)-(4'R,5'S,7'R)-細(xì)鏈格孢烯（3）、4'-表-二氫細(xì)鏈格孢烯A（4）、細(xì)鏈格孢三醇5-甲醚（5）、細(xì)鏈格孢三醇（6）和細(xì)鏈格甲醚孢醇（7）。化合物5和7表現(xiàn)出抗炎活性，半數(shù)抑制濃度（median inhibition concentration，IC₅₀）值分別為（14.80±0.67）μmol/L和（27.76±2.75）μmol/L。結(jié)論 化合物1為聚酮類新化合物，命名為3-甲氧基細(xì)鏈格孢內(nèi)酯B；化合物5有顯著抗炎活性。

Objective To study the secondary metabolites and its biological activities of the endophyties Alternaria malvae derived from the roots of Salvia miltiorrhiza. Methods Preparative high-performance liquid chromatography, along with additional chromatographic techniques including normal-phase column chromatography, MPLC-ODS, MCI, and Sephadex LH-20, was employed to isolate and purify the secondary metabolites. NMR and mass spectrometry were then used to characterize the individual compounds. Results A total of seven compounds were isolated from the rice fermentation of A. malvae and identified as 3-methoxyalternolide B (1), 4'-epialtenuene (2), (＋)-(4'R,5'S,7'R)-altenuene (3), 4'-epi-dihydroaltenuene A (4), alternariol 5-methyl ether (5), alternariol (6) and altenuisol (7). Compound 1 was a new compound, Compounds 5 and 7 exhibited anti-inflammatory activity with IC₅₀ values of (14.8±0.67) μmol/L and (27.76±2.75) μmol/L, respectively. Conclusion Compound 1 is a new polyketone compound named 3-methoxyalternolide B. Compound 5 has significant anti-inflammatory activity.

R284.1

安徽省高校學(xué)科（專業(yè)）帶頭人培育項目（DTR2023026）;安徽省高等學(xué)校科學(xué)研究重點(diǎn)項目（2022AH050489）;中藥研究與開發(fā)安徽省重點(diǎn)實(shí)驗(yàn)室開放課題（AKLPDCM202305）