目的 研究鷹爪花Artabotrys hexapetalus中的化學(xué)成分及其細(xì)胞毒活性。方法 運(yùn)用多種色譜方法，對(duì)鷹爪花莖和根的95%乙醇提取物進(jìn)行分離純化，獲得的化合物用核磁共振（NMR）、高分辨質(zhì)譜（HRESIMS）和電子圓二色譜等手段進(jìn)行結(jié)構(gòu)鑒定。采用CCK-8法評(píng)估化合物對(duì)3種腫瘤細(xì)胞（人肝癌HepG2細(xì)胞、人非小細(xì)胞肺癌A549細(xì)胞和人結(jié)腸癌HCT116細(xì)胞）增殖的影響。結(jié)果 從鷹爪花95%乙醇提取物中分離得到8個(gè)化合物，其中1個(gè)新阿樸菲和7個(gè)已知的酰胺類生物堿，分別鑒定為(E)-(6aS,7R)-7-羥基-3-甲氧基-N-丙烯醛基-1,2-亞甲二氧基氧化阿樸菲堿（1）、trans-N-p-coumaroyl tyramine（2）、N-trans-3-hydroxy-4-methoxycinna moyltyramine（3）、N-trans-feruloyltyramine（4）、(E)-3-(4-hydroxy-3-methoxyphenyl)-N-phenethyl-acrylamide（5）、northalifoline（6）、tribulusamide A（7）、大麻酰胺F（8）?；衔?b>7和8能顯著抑制HepG2細(xì)胞增殖，細(xì)胞半數(shù)抑制濃度（median inhibition concentration，IC₅₀）分別是（30.52±0.93）μmol/L和（16.26±1.97）μmol/L。結(jié)論 化合物1為新化合物，命名為鷹爪花堿E（hexapetalusine E）；化合物3～8為首次從鷹爪花屬植物中分離得到，化合物7和8對(duì)HepG2細(xì)胞表現(xiàn)出明顯的細(xì)胞毒性。

Objective To investigate the chemical constituents and cytotoxic activities of Artabotrys hexapetalus. Methods The 95% ethanol extracts of the stems and roods of A. hexapetalus were purified on the basis of several chromatographic methods. The structural identification of isolated compounds were based on NMR, HRESIMS and ECD calculation. The cytotoxic activity against three types of tumor cells (HepG2, A549, HCT116) were tested by the CCK-8 methods.Results One undescribed aporphine alkaloid and seven previously reported amide alkaloids were isolated from the 95% ethanol extracts and elucidated as (E)-(6aS,7R)-7-hydroxy-3-methoxy-N-acrylaldehyde-1,2-methylenedioxy oxoaporphine alkaloid (1), trans-N-p-coumaroyltyramine (2), N-trans-3-hydroxy-4-methoxycinnamoyltyramine (3), N-trans-feruloyl-tyramine (4), (E)-3-(4-hydroxy-3-methoxyphenyl)-N-phenethyl-acrylamide (5), northalifoline (6), tribulusamide A (7), and cannabisin F (8). Compounds 7 and 8 showed significantly cytotoxic activity against HepG2 cells with IC₅₀ values of (30.52 ±0.93) μmol/L and (16.26 ±1.97) μmol/L, respectively. Conclusions Compound 1 was an unreported compound named hexapetalusine E, and compounds 3—8 were isolated for the first time from the genus Artabotrys. Compounds 7 and 8 showed significant cytotoxicity against HepG2 cells.

R284.1

國(guó)家自然科學(xué)基金面上項(xiàng)目（32271523）;2022年銀川市學(xué)術(shù)技術(shù)帶頭人儲(chǔ)備工程項(xiàng)目（銀人才發(fā)[2022]37號(hào)）