目的 研究佛手Citrus medica var. sarcodactylis干燥果實(shí)的化學(xué)成分。方法 應(yīng)用正相硅膠、Sephadex LH-20凝膠柱色譜、ODS以及半制備型HPLC等色譜技術(shù)進(jìn)行系統(tǒng)分離純化，并且通過(guò)核磁共振波譜、質(zhì)譜等技術(shù)鑒定單體化合物的結(jié)構(gòu)。結(jié)果 從佛手95%乙醇提取物中共分離得到26個(gè)化合物，分別鑒定為大黃酚（1）、大黃素甲醚（2）、大黃素（3）、蘆薈大黃素（4）、卡枯醇（5）、2-甲氧基-4,5-亞甲二氧基苯丙酮（6）、methoxylatifolone（7）、和厚樸酚（8）、牛蒡子苷元（9）、羅漢松樹(shù)脂酚（10）、表松脂酚（11）、lappaol F（12）、arctium B（13）、isolappaol A（14）、lappaol A（15）、3-(1,3-benzodioxol-5-yl)-1,2-propanediol（16）、lappaol B（17）、丁香脂素（18）、吳茱萸次堿（19）、墻草堿（20）、asaroidoxazine B（21）、蔓荊子黃素（22）、2-(3,4-dimethoxyphenyl)-3,5,8-trihydroxy-7-methoxy-4H-1-benzopyran-4-one（23）、2-(3,4-dimethoxyphenyl)-5,6,7-trihydroxy-3-methoxy-4H-1-benzopyran-4-one（24）、(22E,24R)-5α,8α,-過(guò)氧麥角甾-6,9,22-三烯-3β-醇（25）和過(guò)氧麥角甾醇（26）。結(jié)論 所有化合物均為首次從佛手中分離得到，為佛手的開(kāi)發(fā)利用提供一定的物質(zhì)基礎(chǔ)。

Objective To study the chemical constituents from the fruits of Citrus medica. var. sarcodactylis. Methods The chemical constituents were isolated and purified by silica gel, Sephadex LH-20 gel column chromatography, ODS and semi-preparation HPLC. The structures of all isolates were determined on the basis of NMR and MS analysis. Results A total of 26 compounds were isolated from the 95% EtOH extract of C. medica var. sarcodactylis, which were identified as chrysophanic acid (1), physcion (2), emodin (3), aloeemodin (4), kakuol (5), 2-methoxy-4,5-methylenedioxypropiophenone (6), methoxylatifolone (7), honokiol (8), arctigenin (9), matairesinol (10), epipinoresinol (11), lappaol F (12), arctium B (13), isolappaol A (14), lappaol A (15), 3-(1,3-benzodioxol-5-yl)-1,2-propanediol (16), lappaol B (17), syringaresinol (18), rutaecarpine (19), pellitorine (20), asaroidoxazine B (21), casticin (22), 2-(3,4-dimethoxyphenyl)-3,5,8-trihydroxy-7-methoxy-4H-1-benzopyran-4-one (23), 2-(3,4-dimethoxyphenyl)-5,6,7-trihydroxy-3-methoxy-4H-1-benzopyran-4-one (24), (22E,24R)-5α,8α,-epidioxyergosta-6,9,22-triene-3β-ol (25) and ergosterol peroxide (26). Conclusion All compounds were isolated from the fruits of C. medica var. sarcodactylis for the first time. This study provides certain study basis for the utilization of C. medica. var. sarcodactylis.

R284.1

廣州中醫(yī)藥大學(xué)“雙一流”與高水平大學(xué)學(xué)科協(xié)同創(chuàng)新團(tuán)隊(duì)項(xiàng)目（2021xk31）