目的 探究杧果葉Mangiferae Indicae Folium水提物和醇提物的消滯功效差異，并建立不同品種杧果葉2種提取物的HPLC指紋圖譜，再利用網(wǎng)絡藥理學和多成分定量法對2種提取物中的消滯成分進行研究。方法 制備不同品種杧果葉醇提物和水提物；使用幼齡SD大鼠探究杧果葉2種提取物的“行氣消滯”功效；采用默克STAR RP-18e（250 mm×4.6 mm，5 μm）色譜柱，乙腈-0.1%磷酸水溶液為流動相，梯度洗脫，柱溫30 ℃，體積流量0.8 mL/min，檢測波長216 nm，繪制8種杧果葉水提物和8種杧果葉醇提物樣品的HPLC指紋圖譜，篩選共有峰，通過層次聚類分析（hierarchical clustering analysis，HCA）、主成分分析（principal component analysis，PCA）、網(wǎng)絡藥理學等方法評價杧果葉水提物和醇提物中消滯成分；并對可測消滯成分進行定量測定。結(jié)果 杧果葉2種提取物都具有消滯功效，杧果葉醇提物有利于幼齡SD大鼠體質(zhì)量的增加和胃排空能力的增強，杧果葉水提物可以顯著提高胃腸道蠕動能力。16種杧果葉提取物樣品的HPLC指紋圖譜共匹配出14個共有峰，指紋圖譜相似度在0.646～0.984，HCA和PCA結(jié)果顯示，杧果葉提取物可以聚類分析；網(wǎng)絡藥理學預測分析結(jié)果顯示，杧果葉中發(fā)揮消滯功效的化合物有14種，使用對照品對比鑒定出楊梅素-3-O-β-D-吡喃半乳糖苷（10號峰）、木犀草素-7-O-葡萄糖醛酸苷（11號峰）、槲皮苷（12號峰）和槲皮素（14號峰）4種成分；杧果葉醇提物和水提物中4種可測成分楊梅素-3-O-β-D-吡喃半乳糖苷、木犀草素-7-O-葡萄糖醛酸苷、槲皮苷和槲皮素的質(zhì)量分數(shù)分別為0.081%～0.297%、0.254%～3.104%、0.005%～0.056%、0.029%～0.314%。結(jié)論 杧果葉提取物具有消滯功效，用HPLC指紋圖譜結(jié)合網(wǎng)絡藥理學和多成分分析可以評估和篩選杧果葉中消滯成分的差異性，為杧果葉質(zhì)量控制提供了參考。

Objective The differences of ethanol and water extracts from Mangguoye (Mangiferae Indicae Folium, MIF) in eliminating stagnation effects were explored, then HPLC fingerprints of MIF ethanol and water extracts of different varieties were established, and network pharmacology and multi-component quantification were used to investigate the differential components of eliminating stagnation in two MIF extracts. Methods Ethanol and water extracts from MIF of different varieties were prepared; the effects of “promoting qi and eliminating stagnation” of two MIF extracts were explored by suckling SD rats. Then a Merck STAR RP-18e (250 mm×4.6 mm, 5 μm) column was used with acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase for gradient elution, with column temperature of 30 ℃, volume flow rate of 0.8 mL/min, detection wavelength of 216 nm, and the fingerprint of eight MIF water extracts and eight MIF ethanol extracts was drawn to screen the common peaks. The hierarchical cluster analysis (HCA), principal component analysis (PCA) and network pharmacology methods were used to evaluate the differential components of eliminating stagnation in MIF under different treatment methods. And the content of four measurable effective components was determined. Results Two MIF extracts all had the ffects of eliminating stagnation. MIF ethanol extract was beneficial for the increase of suckling SD rats weight and gastric emptying rate, while its water extract could improve the small intestine propulsion rate and gastric emptying rate. Fourteen shared peaks were identified from the MIF fingerprint profile, with similarities between 0.646 and 0.984 for each batch. The results of HCA and PCA showed that MIF extracts could be clustered analysis. Fourteen compounds were predicted and analyzed in MIF with eliminating stagnation effects by network pharmacology, and four compounds were identified as common peaks in the fingerprint by comparison with the reference substances, namely myricetin 3-O-β-D-galactopyranoside, luteolin-7-O-glucuronide, quercitrin and quercetin. The contents of myricetin 3-O-β-D-galactopyranoside, luteolin-7-O-glucuronide, quercitrin and quercetin in MIF were 0.081%—0.297%, 0.254%—3.104%, 0.005%—0.056%, 0.029%—0.314%, respectively. Conclusion MIF extracts have eliminating stagnation effect, HPLC fingerprint combined with network pharmacology and chemometrics can be used to assess and screen the differences of eliminating stagnation components in MIF, which provides provide a basis for its quality control.

R283.6

廣西自然科學基金項目（2024GXNSFBA010168）;廣西教育廳中青年能力提升項目（2024KY0319）;農(nóng)作物廢棄物功能成分研究協(xié)同創(chuàng)新中心資助課題（CICAR2023-P1）;中醫(yī)藥生物新材料關(guān)鍵技術(shù)研究與智能裝備開發(fā)學科交叉創(chuàng)新團隊（GZKJ2303）