目的 基于轉(zhuǎn)錄組數(shù)據(jù)篩選鑒定分析甘松Nardostachys jatamansi CONSTANS（CO）轉(zhuǎn)錄因子家族。方法 運(yùn)用生物信息學(xué)技術(shù)對甘松植物的CO轉(zhuǎn)錄因子家族成員展開綜合分析，深入探究其蛋白的理化特性、基因序列結(jié)構(gòu)、亞細(xì)胞位置、導(dǎo)肽和信號肽的預(yù)測，以及跨膜結(jié)構(gòu)域的識別。此外，通過構(gòu)建系統(tǒng)進(jìn)化樹，進(jìn)一步揭示這些基因家族成員間的進(jìn)化關(guān)系。結(jié)果 在甘松的數(shù)據(jù)庫中經(jīng)過篩選、鑒定，最終得到10個CO轉(zhuǎn)錄因子，氨基酸長度為316～440 aa，相對分子質(zhì)量在35 561.67～48 171.96，理論等電點范圍為5.11～8.63，主要在細(xì)胞核中表達(dá)，均含有B-box和CCT結(jié)構(gòu)域，均不含跨膜結(jié)構(gòu)域和信號肽。構(gòu)建系統(tǒng)進(jìn)化樹明確了各亞族的成員分布：第Ⅰ亞族包含2個成員，第Ⅱ亞族增至3個成員，而第Ⅲ亞族則有5個成員，這一發(fā)現(xiàn)為進(jìn)一步探討各亞族間的進(jìn)化關(guān)系及特性差異提供了重要基礎(chǔ)。結(jié)論 利用了生物信息學(xué)的手段，全面鑒定并分析了甘松CO轉(zhuǎn)錄因子家族的理化性質(zhì)、蛋白結(jié)構(gòu)、系統(tǒng)發(fā)育關(guān)系等，對甘松CO轉(zhuǎn)錄因子家族功能的詳盡研究，為后續(xù)的深入探索提供了寶貴的參考。此外，也為理解甘松藥用成分合成的調(diào)控機(jī)制提供了基礎(chǔ)，并為未來培育出更高品質(zhì)的甘松品種提供了科學(xué)依據(jù)。

Objective According to the transcriptome data, the CONSTANS (CO) transcription factor family members were identified and analyzed in Nardostachys jatamansi. Methods The bioinformatic technology was used to analyze the CO transcription factor family members of n N. jatamansi plants, including the physicochemical properties of proteins, gene sequence architecture analysis, subcellular location, peptides and signaling peptides prediction, and identification of transmembrane domains. The phylogenetic tree was constructed to reveal the evolutionary relationship between them. Results A total of 10 members of the CO transcription factor family were screened and identified based on the N. jatamansi database. The length of amino acids was 316—440 aa, the relative molecular weight was 35 561.67—48 171.96, and the theoretical range of isoelectric point was 5.11—8.63. They are mainly expressed in the nucleus, contain B-box and CCT domains, and don’t contain transmembrane domains and signal peptides. The phylogenetic tree was constructed to clarify the distribution of members of each subfamily: Subfamily I contains two members, subfamily II has three members, and subfamily III has five members. This discovery provides an important basis for further exploration of the evolutionary relationship and characteristic differences among subfamilies. Conclusion The physicochemical properties, protein structure and phylogenetic relationships of CO family transcription factors were identified and analyzed by means of bioinformatics. The detailed study on the function of CO family transcription factors provided a valuable reference for further exploration. In addition, this study also provides a basis for understanding the regulatory mechanism of medicinal ingredient synthesis of N. jatamansi, and provides a scientific basis for higher-quality breeding in the future.

R286.2

四川省自然科學(xué)基金資助項目（2025ZNSFSC1124）;西南民族大學(xué)青藏高原研究首批科技創(chuàng)新團(tuán)隊（2024CXTD04）;西南民族大學(xué)人才科研項目（RQD2021110）;中央高?；究蒲袠I(yè)務(wù)費(fèi)專項基金項目（ZYN2024189）