目的 對(duì)直立百部Stemona sessilifolia塊根的化學(xué)成分進(jìn)行分離鑒定。方法 采用硅膠、Sephadex LH-20、ODS等柱色譜及制備型高效液相等方法進(jìn)行分離純化；根據(jù)化合物的波譜數(shù)據(jù)（MS、¹H-與¹³C-NMR）鑒定其結(jié)構(gòu)；利用CCK-8法及Griess法測(cè)定化合物的細(xì)胞毒活性及抗炎活性（NO含量）。結(jié)果 從直立百部塊根的乙醇提取物中分離得到8個(gè)酚類化合物，分別鑒定為4-氯-3-甲氧基-5-甲基苯酚-1-O-β-D-呋喃芹糖基-(1→6)-β-D-吡喃葡萄糖苷（1）、4-氯-3-甲氧基-5-甲基苯基- 1-O-α-L-吡喃鼠李糖基-(1→6)-β-D-吡喃葡萄糖苷（2）、3,4,5-三甲氧基苯基-1-O-β-D-吡喃葡萄糖苷（3）、刺五加苷B（4）、腫柄雪蓮苷（5）、(＋)-異落葉松樹脂醇-3α-O-β-D-吡喃葡萄糖苷（6）、去氫-δ-生育酚（7）與去氫-γ-生育酚（8）。結(jié)論 化合物1為1個(gè)新化合物，化合物2～6為首次從百部屬植物中分離得到，7和8為首次從直立百部中分離得到；化合物3、4、7和8對(duì)RAW 264.7細(xì)胞沒有明顯的細(xì)胞毒性，存活率均大于85%，其具有弱的抗炎活性，在20 μmol/L下對(duì)脂多糖誘導(dǎo)的RAW 264.7細(xì)胞炎癥因子NO釋放的抑制率在20%～50%。

Objective To study chemical constituents in the Stemona sessilifolia roots with their anti-inflammatory activities. Methods Compounds were isolated and purified by various chromatography, including silica gel, ODS, Sephadex LH-20 and preparative HPLC. Their structures were elucidated by spectroscopic data analysis. Cytotoxicity and anti-inflammatory activities were evaluated by CCK-8 and Griess experiments. Results Eight compounds were obtained from the tuber roots of S. sessilifolias and identified as 4-chloro-3-methoxy-5-methylphenol-1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (1), 4-chloro-3-methoxy-5-methylphenyl-1-O-α-L-rhanopyranosyl-(1→6)-β-D-glucopyranoside (2), 3,4,5-trimethoxyphenyl-1-O-β-D-glucopyranoside (3), eleutheroside B (4), conicaoside (5), (+)-isolariciresinol-3α-O-β-D-glucopyranoside (6), dehydro-δ-tocopherol (7) and dehydro-γ-tocopherol (8). Conclusion Compound 1 is a new natural product, compounds 2―6 were isolated from Stemona genus for the first time, and compounds 7 and 8 were obtained firstly from S. sessilifolias. Compounds 3, 4, 7 and 8 showed no cytotoxicity on RAW 264.7 cells and weak anti-inflammatory activities all with 20%―50% NO inhibition at 20 μmol/L.

R284.1

國家中醫(yī)藥管理局科技司−山東省衛(wèi)生健康委員會(huì)共建中醫(yī)藥科技項(xiàng)目(GZY-KJS-SD-2023-087)