目的 闡明番瀉葉Sennae Folium的化學(xué)成分，并對主要化合物進(jìn)行定量分析，為番瀉葉質(zhì)量評價(jià)提供依據(jù)。方法 采用超高效液相色譜-四極桿-靜電場軌道阱高分辨質(zhì)譜（UHPLC-Q-exactive-orbitrap-MS）負(fù)離子模式對番瀉葉中主要化學(xué)成分進(jìn)行快速鑒定；采用UPLC-PDA法對6個(gè)主要化合物進(jìn)行定量分析，并應(yīng)用于多批次樣品測定。結(jié)果 番瀉葉鑒定了29個(gè)化合物，其中蒽醌類化合物12個(gè)、黃酮類化合物11個(gè)、二苯甲酮類化合物2個(gè)及萘類化合物4個(gè)；測定了20批番瀉葉樣品中6個(gè)主要成分（新西蘭牡荊苷、蘆薈大黃素-8-O-葡萄糖苷、番瀉苷A、番瀉苷B、番瀉苷C、丁內(nèi)未利葡萄糖苷）的含量，不同批次中各化合物含量存在差異。結(jié)論 對番瀉葉中化學(xué)成分進(jìn)行快速鑒定和主要成分定量分析，為番瀉葉的質(zhì)量評價(jià)提供依據(jù)。

Objective To clarify the chemical components and quantify the main components, so as to provide the basis for the quality evaluation of Fanxieye (Sennae Folium). Methods UHPLC-Q-Exactive-Orbitrap-MS was employed to quickly identify the main chemical components of Sennae Folium in negative ion mode. Furthermore, the quantitative analysis of six main chemical components was accomplished by ultra performance liquid chromatography with photo-diode array (UPLC-PDA) detection in Sennae Folium. Results A total of 29 components were identified, including 12 anthraquinones, 11 flavonoids, two benzophenones and four naphthalenes. The content of six main components (vicenin 2, aloe emodin-8-O-glucoside, sennoside A, sennoside B, sennoside C, and tinnevellin glucoside) were determined in 20 batches Sennae Folium, which varied obviously in different batches. Conclusion The rapid identification of chemical components and the quantitative analysis of six components were successfully accomplished in Sennae Folium, which provided the basis for the quality evaluation of Sennae Folium.

R286.2

國家中醫(yī)藥管理局中藥標(biāo)準(zhǔn)化項(xiàng)目（ZYBZH-C-JX-38）