目的 對甘肅引種栽培的不同品種金銀花Lonicerae Japonicae Flos進行品質(zhì)分析，為金銀花品種選育和產(chǎn)業(yè)化開發(fā)提供新思路。方法 采用ITS2條形碼序列分析11個不同品種金銀花的種間差異；采用HPLC建立金銀花特征圖譜，并對13個樣品的指標(biāo)成分進行含量測定；采用超高效液相色譜-四級桿飛行時間質(zhì)譜（UPLC-QTOF-MS/MS）進行非靶向代謝組學(xué)分析。結(jié)果 通過ITS2序列對比分析發(fā)現(xiàn)，金花3號、豫金2號、豫花1號和華金6號不同品種間核苷酸序列位點堿基發(fā)生較大突變，其中華金6號有24處堿基差異。根據(jù)HPLC特征圖譜，指認了特征成分綠原酸、異綠原酸A和異綠原酸C。不同品種金銀花綠原酸、異綠原酸A、異綠原酸C和木犀草苷的含量存在差異，其中北花1號、華金6號、封花1號、金花3號與豫金1號金銀花的酚酸類成分及木犀草苷含量均顯著高于《中國藥典》2020年版的規(guī)定。不同品種金銀花代謝組學(xué)分析共篩選出21個差異代謝物，包括異綠原酸、山柰酚-O-蕓香糖苷等。結(jié)論 北花1號、華金6號、封花1號、金花3號與豫金1號金銀花作為重點品種，可結(jié)合生產(chǎn)實踐進行推廣示范研究。系統(tǒng)分析了不同品種金銀花中主要有效成分的異同，為金銀花品種鑒定、質(zhì)量控制和生產(chǎn)利用提供了科學(xué)依據(jù)。

Objective In order to offer new insights for the breeding and industrialization of Jinyinhua (Lonicerae Japonicae Flos, LJF), the comprehensive quality evaluation of different varieties of LJF cultivated in Gansu Province was carried out. Methods ITS2 barcode sequence was used to analyze the interspecific differences of 11 different varieties of LJF medicinal materials. The characteristic chromatogram of LJF was established by high performance liquid chromatography (HPLC), and the content of indicator components in 13 samples was determined. Un-targeted metabolomics analysis was performed using ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS). Results Through ITS2 sequence alignment, it was found that there were significant mutations in nucleotide sequence sites among different varieties of Jinhua 3, Yujin 2, Yuhua 1, and Huajin 6, with 24 nucleotide differences observed in Huajin 6. Chlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C were identified in the HPLC characteristic chromatograms. Different varieties of LJF showed significant differences in the content of chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, and luteoloside. The phenolic acid and luteolin content of Beihua 1, Huajin 6, Fenghua 1, Jinhua 3, and Yujin 1 were significantly higher than those specified in the 2020 edition of the Chinese Pharmacopoeia. Metabolomics analysis showed a total of 21 differential metabolites, including isochlorogenic acid, kaempferol-O-rutinoside. Conclusion Based on comprehensive analysis, Beihua 1, Huajin 6, Fenghua 1, Jinhua 3, and Yujin 1 could be selected as key varieties for the further production practice. This study systematically evaluated the similarities and differences of the main active components in different varieties of LJF, which provided scientific basis for variety identification, quality control and production and utilization of LJF in Gansu Province.

R286.12

甘肅省科技計劃項目(22CX8NA003)；國家藥監(jiān)局中藥材及飲片質(zhì)量控制重點實驗室項目(2024GSMPA-KL08）