目的 利用光照處理、低溫處理與赤霉素A3（gibberellic acid，GA3）處理探究月見草Oenothera parviflora抽薹的影響因素，通過轉錄組篩選GA3處理條件下的抽薹開花相關基因。方法 分別利用0、200、400、600、800 mg/L GA3處理3 d和10 d，并測定月見草的的抽薹率，進一步對噴施GA3前后月見草的莖尖進行轉錄組分析，篩選抽薹開花相關差異表達基因。結果 發(fā)現(xiàn)，僅有GA3處理月見草營養(yǎng)體的抽薹效果最為顯著，其中，600 mg/L GA3處理條件下月見草的抽薹率最高，抽薹率為96.67%。對其進行轉錄組分析發(fā)現(xiàn)，GA3噴施后有1 626個基因差異表達，其中有863個基因表達上調，763個基因顯著下調。通過基因注釋信息與擬南芥同源基因比較發(fā)現(xiàn)，抽薹開花相關基因OpTEM1、OpCO、OpFD、OpAGL19、OpAGL42、OpAP1、OpRGL1、OpSPL5可能在GA3作用下參與調控月見草抽薹開花調控。利用熒光定量進一步確認差異基因的表達模式，其中ObCO、ObAP1、ObAGL19、ObAGL42、ObSPL5基因顯著上調，ObTEM1、ObFD、ObRGL1基因顯著下調，結果與轉錄組一致。結論 外源GA3可以顯著促進月見草的抽薹開花進程，TEM1、CO、FD、AGL19、AGL42、AP1、RGL1、SPL5等基因可能參與調控月見草的抽薹開花進程。可以揭示月見草植物抽薹特性及其植株發(fā)育動態(tài)特征，對進一步明確月見草的抽薹機制和指導月見草生產(chǎn)實踐具有重要意義。

Objective The influencing factors of bolting in Oenothera parviflora were investigated by light treatment, low-temperature treatment and gibberellic acid (GA3), and the genes related to bolting and flowering under GA3 treatment were screened by transcriptome. Method Different concentrations of GA3 with 0, 200, 400, 600, 800 mg/L were applied for treatment 3 d and 10 d, respectively, and the bolting rate of Oenothera parviflora were determined. Further transcriptome analysis was conducted on the stem apex before and after GA3 spraying to screen for differentially expressed genes related to bolting and flowering. Results varying photoperiod, light quality, low temperature, and treatments with GA3 indicated that GA3 application had the most significant impact on the bolting time. Among the different treatments, 600 mg/L GA3 solution led to the highest bolting rate at 96.67%. Transcriptional profiling showed that following GA3 treatment, 1 626 genes demonstrated differential expression, with 863 upregulated and 763 downregulated genes. Compared to homologous genes in Arabidopsis thaliana, suggested that genes such as OpTEM1, OpCO, OpFD, OpAGL19, OpAGL42, OpAP1, OpRGL1, OpSPL5, which are related to bolting and flowering, might play a role in regulating bolting and flowering time under GA3 treatment. Conclusion These findings shed light on the bolting characteristics and developmental dynamics of the Oenothera parviflora, which is crucial for elucidating the bolting mechanisms and guiding practical cultivation strategies.

R286.2

國家現(xiàn)代農業(yè)產(chǎn)業(yè)技術體系遼寧創(chuàng)新團隊建設