目的 利用光照處理、低溫處理與赤霉素A3（gibberellic acid，GA3）處理探究月見草Oenothera parviflora抽薹的影響因素，通過轉(zhuǎn)錄組篩選GA3處理?xiàng)l件下的抽薹開花相關(guān)基因。方法 分別利用0、200、400、600、800 mg/L GA3處理3 d和10 d，并測定月見草的的抽薹率，進(jìn)一步對噴施GA3前后月見草的莖尖進(jìn)行轉(zhuǎn)錄組分析，篩選抽薹開花相關(guān)差異表達(dá)基因。結(jié)果 發(fā)現(xiàn)，僅有GA3處理月見草營養(yǎng)體的抽薹效果最為顯著，其中，600 mg/L GA3處理?xiàng)l件下月見草的抽薹率最高，抽薹率為96.67%。對其進(jìn)行轉(zhuǎn)錄組分析發(fā)現(xiàn)，GA3噴施后有1 626個(gè)基因差異表達(dá)，其中有863個(gè)基因表達(dá)上調(diào)，763個(gè)基因顯著下調(diào)。通過基因注釋信息與擬南芥同源基因比較發(fā)現(xiàn)，抽薹開花相關(guān)基因OpTEM1、OpCO、OpFD、OpAGL19、OpAGL42、OpAP1、OpRGL1、OpSPL5可能在GA3作用下參與調(diào)控月見草抽薹開花調(diào)控。利用熒光定量進(jìn)一步確認(rèn)差異基因的表達(dá)模式，其中ObCO、ObAP1、ObAGL19、ObAGL42、ObSPL5基因顯著上調(diào)，ObTEM1、ObFD、ObRGL1基因顯著下調(diào)，結(jié)果與轉(zhuǎn)錄組一致。結(jié)論 外源GA3可以顯著促進(jìn)月見草的抽薹開花進(jìn)程，TEM1、CO、FD、AGL19、AGL42、AP1、RGL1、SPL5等基因可能參與調(diào)控月見草的抽薹開花進(jìn)程?？梢越沂驹乱姴葜参锍檗诽匦约捌渲仓臧l(fā)育動(dòng)態(tài)特征，對進(jìn)一步明確月見草的抽薹機(jī)制和指導(dǎo)月見草生產(chǎn)實(shí)踐具有重要意義。

Objective The influencing factors of bolting in Oenothera parviflora were investigated by light treatment, low-temperature treatment and gibberellic acid (GA3), and the genes related to bolting and flowering under GA3 treatment were screened by transcriptome. Method Different concentrations of GA3 with 0, 200, 400, 600, 800 mg/L were applied for treatment 3 d and 10 d, respectively, and the bolting rate of Oenothera parviflora were determined. Further transcriptome analysis was conducted on the stem apex before and after GA3 spraying to screen for differentially expressed genes related to bolting and flowering. Results varying photoperiod, light quality, low temperature, and treatments with GA3 indicated that GA3 application had the most significant impact on the bolting time. Among the different treatments, 600 mg/L GA3 solution led to the highest bolting rate at 96.67%. Transcriptional profiling showed that following GA3 treatment, 1 626 genes demonstrated differential expression, with 863 upregulated and 763 downregulated genes. Compared to homologous genes in Arabidopsis thaliana, suggested that genes such as OpTEM1, OpCO, OpFD, OpAGL19, OpAGL42, OpAP1, OpRGL1, OpSPL5, which are related to bolting and flowering, might play a role in regulating bolting and flowering time under GA3 treatment. Conclusion These findings shed light on the bolting characteristics and developmental dynamics of the Oenothera parviflora, which is crucial for elucidating the bolting mechanisms and guiding practical cultivation strategies.

R286.2

國家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系遼寧創(chuàng)新團(tuán)隊(duì)建設(shè)