目的 探究不同采收期茵陳中化學(xué)成分的動態(tài)變化規(guī)律，為揭示“綿茵陳”和“花茵陳”品質(zhì)形成機(jī)制奠定基礎(chǔ)。方法 以6個不同采收期（S1：幼苗期、S2～S4：營養(yǎng)生長期、S5：開花期、S6：結(jié)果期）茵陳蒿為材料，利用HPLC和分光光度法對主要活性物質(zhì)含量以及體外抗氧化能力進(jìn)行測定與分析。結(jié)果 隨著采收期延長，綠原酸含量呈顯著下降趨勢，在S1時期（“綿茵陳”期）最高5.69 mg/g，而濱蒿內(nèi)酯含量呈顯著增加趨勢，在S5時期（“花茵陳”期）最高3.58 mg/g。多糖含量在S1和S5時期相對較高，酚類和黃酮類含量以及1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）清除率和鐵離子還原/氧化能力（ferric reducing/antioxidant power，F(xiàn)RAP）值均呈“M型”變化趨勢，表現(xiàn)S2、S3和S5時期相對較高。結(jié)論 采收期對茵陳中化學(xué)成分具有顯著影響，為茵陳資源開發(fā)和利用提供理論依據(jù)和有益參考。

Objective To explore the dynamic change rule of chemical compounds in Artemisia capillaris at different harvest stages, and lay the foundation for revealing the formation mechanism of quality in A. capillaris harvested in spring and autumn. Methods The main active substance contents and in vitro antioxidant capacity in A. capillaris harvested at six different stages (S1: seedling stage, S2 to S4: vegetative growth stage; S5: flowering stage; S6: fruiting stage) were determined and analyzed using the HPLC and spectrophotometry. Results With the extension of harvest stage, the chlorogenic acid contents showed a significant decrease and reached the highest value of 5.69 mg/g at the S1 (seedling stage), while the scoparone content showed a significant increase and reached the highest value of 3.58 mg/g at the S5 (flower bud stage). The polysaccharide contents were relatively high at S1 and S5, and the phenolic and flavonoids and phenolic contents as well as DPPH scavenging rate and FRAP value showed a “M” changetrend with a relatively high l level at the S2、S3 and S5. Conclusion There is a significant effect of harvest stage on chemical compounds in A. capillaris, these findings will provide theoretical basis and useful references for resource exploitation and utilization of A. capillaris.

R286.2

國家中藥材產(chǎn)業(yè)技術(shù)體系（CARS-21）；道地藥材生態(tài)種植及質(zhì)量保障項(xiàng)目（202203002）