目的 分析1～12月忍冬Lonicerae japonicae葉中12種活性成分含量的動態(tài)變化，評價不同采收期忍冬葉質(zhì)量。方法 采用UPLC法檢測每月采摘的忍冬葉中綠原酸、當(dāng)藥苷、斷氧化馬錢子苷、異槲皮苷等12種活性成分的含量，繪制動態(tài)變化曲線，分析成分含量變化，并結(jié)合聚類分析、主成分分析（principal component analysis，PCA）、正交偏最小二乘判別分析（orthogonal partial least squares-discriminant analysis，OPLS-DA），構(gòu)建不同采收期忍冬葉中12種活性成分的含量差異性模型，綜合評價忍冬葉的質(zhì)量。結(jié)果 UPLC法測得3、2月忍冬葉中化學(xué)成分的含量較高，4、12、1月次之。根據(jù)所測每月忍冬葉中活性成分的含量，聚類分析、PCA、OPLS-DA分析一致表明，忍冬葉被分為4類，12、1、2月為一類，5、6、7、8、9、10、11月為一類，3月為一類，4月為一類。進(jìn)一步的多元統(tǒng)計分析顯示，綠原酸、異綠原酸A、木犀草苷或是導(dǎo)致忍冬葉質(zhì)量差異的關(guān)鍵成分。結(jié)論 忍冬葉中12種活性成分含量一年中變化顯著，3、2、4、12、1月忍冬葉的質(zhì)量較好，綠原酸、異綠原酸A、木犀草苷可以作為忍冬葉質(zhì)控的關(guān)鍵成分。結(jié)合忍冬種植模式，12、1、2月為忍冬葉最佳采收期。研究結(jié)果為忍冬葉的質(zhì)量控制及評價提供方法和依據(jù)，也為忍冬葉的合理開發(fā)利用提供支持。

Objective To evaluate the quality of Lonicerae japonicae leaves picked from January to December based on the dynamic changes of 12 active ingredients. Methods An ultra-high performance liquid chromatography (UPLC) method was used to determinethe contents of 12 active ingredients such as chlorogenic acid, sweroside, secoxyloganin and isoquercetin in Lonicerae japonicae leaves picked every month. Dynamic change curves were plotted to analyze the content change of each ingredient. To comprehensively evaluate the quality of Lonicerae japonicae leaves harvested at different periods, clustering analysis, principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were respectively constructed to compare the contents of 12 active components. Results The 12 active ingredients changed significantly throughout the year. The total contents of chemical components in Lonicerae japonicae leaves picked in March and February were the highest, followed by April, December and January. According to the content of active ingredients in Lonicerae japonicae leaves measured each month, the results of cluster analysis, PCA and OPLS-DA consistently indicated that Lonicerae japonicae leaves were divided into four categories, one in December, January and February, one in May, June, July, August, September, October and November, one in March and one in April. Further multivariate statistical analysis showed that chlorogenic acid, isochlorogenic acid A and luteolin might be the key components causing the quality difference of Lonicerae japonicae leaves. Conclusion The contents of 12 active ingredients in Lonicerae japonicae leaves picked in different months were significantly different. The quality of Lonicerae japonicae leaves was better in March, February, April, December and January. Chlorogenic acid, isochlorogenic acid A and luteolin could be used as key components for quality control of Lonicerae japonicae leaves. In combination with the planting pattern of Lonicerae japonicae, December, January and February were the best harvesting periods for the leaves. This study will provide methods and basis for the quality control and evaluation of the Lonicerae Japonicae leaves, and also offers support for their rational development and utilization.

R286.2

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